Diagnostic oligonucleotides

High-quality primers, probes, and specialized oligos for diagnostic applications

We provide high-purity oligonucleotides for demanding molecular diagnostic applications.

From research and development to regulated production environments, our oligonucleotides help power precise and accurate diagnostic results in qPCR, NGS, and other molecular testing platforms.

Our diagnostic oligos support:

  • PCR and qPCR primers and probes
  • Next-Generation Sequencing (NGS) workflows
  • Isothermal amplification technologies (LAMP, RPA, etc.)
  • Molecular beacons and other specialized formats
  • Single cell sequencing and other advanced applications

Case Study: YouSeq Partnership

Our partnership with YouSeq demonstrates how ATDBio's high-quality oligonucleotides enable rapid development and deployment of cutting-edge molecular diagnostic solutions. YouSeq has built a reputation for developing sophisticated molecular diagnostic tests with remarkable speed and precision, powered by our oligonucleotides.

Read the full case study

ATDBio offers a comprehensive range of modifications for diagnostic oligonucleotides:

From standard fluorescent labels to specialized chemical modifications, our capabilities span the full spectrum of diagnostic requirements.

Fluorescent Labels & Quenchers

We offer a wide range of fluorophores and quenchers for qPCR probes and other fluorescence-based detection methods.

These include:

  • 5'-FAM
  • 5'-HEX, 5'-TET, 5'-JOE
  • 5'-ROX
  • 3'-TAMRA
  • Cy3
  • Cy5
  • 3'-BHQ1, 3'-BHQ2
  • Internal quenchers for molecular beacons

Functional Modifications & Sequence Optimizations

We offer a comprehensive range of modifications to enhance the functionality and performance of your oligonucleotides, including:

  • 3'-phosphate or 3'-inverse dT to prevent extension by DNA polymerase
  • 5'-phosphate for ligation in NGS library preparation
  • Biotin modifications for capture/enrichment applications
  • Amino linkers and click chemistry moieties for conjugation
  • LNAs to increase duplex stability and specificity
  • Abasic sites to reduce secondary structure
  • Spacers for flexibility and improved performance
  • Degenerate bases for detecting multiple sequence variants
  • Phosphorothioates for increased nuclease resistance
  • Epigenetic modifications for specialized applications

Custom Solutions

We understand that diagnostic development often requires specialized oligos that don't fit standard offerings. Our team has extensive experience in creating custom solutions for unique diagnostic challenges.

From complex modification patterns to unusual sequence formats, we're ready to collaborate on your novel diagnostic approaches. Contact us to discuss your specific requirements.

Discuss your requirements

Why choose ATDBio for your diagnostic oligonucleotides?

Exceptional Purity

High purity oligos are essential to prevent non-specific amplification and hybridization. Our purification process employs rigorous analytical methods with optimized long gradients to accurately determine purity and yield to ensure each oligonucleotide meets the necessary standards for your diagnostic applications.

Batch-to-Batch Consistency

We maintain rigorous quality control processes to ensure exceptional consistency between batches. This is crucial for diagnostic applications where reliable, reproducible results are essential, including ML-trained diagnostic platforms. Our diagnostic partners rely on us to deliver consistent, ready-to-use oligos with purity and yield parameters that fall within a tightly controlled range every single time.

Rapid Lead Times

We understand that time is critical in diagnostics development. Our streamlined processes allow us to begin production as quickly as possible after ordering, with rapid turnaround times to support your development timelines and accelerate your pipeline.

Regulatory Compliance

Our oligonucleotide synthesis processes adhere to strict quality standards, with comprehensive documentation and ISO 9001 certification to support your regulatory requirements. This commitment to quality ensures the highest standards in every oligo we produce for your diagnostic products.

Minimal Cross-Contamination

Our closed-column synthesis process and rigorous cleaning protocols between samples minimize the risk of cross-contamination, which is crucial for sensitive qPCR and NGS applications where even minimal contamination can compromise results.

Proven Track Record

We've supplied oligonucleotides for millions of diagnostic tests, and partnered with diagnostic companies, government organizations, and research institutions across the globe. Our experience has helped partners rapidly respond to an urgent global health crisis and continues to support diagnostic innovation worldwide.

Case Study: COVID-19 Response

During the COVID-19 pandemic, we scaled up our production capacity more than 10-fold to meet the urgent need for diagnostic test components. We supplied primers and probes for tens of millions of COVID-19 tests, working closely with diagnostic companies, government organizations, and research institutions.

Read the full case study

Your partner for diagnostic oligonucleotide synthesis.

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